UC Davis, Agilent Collaborate on Rapid Food Safety Test

SANTA CLARA, Calif.Agilent Technologies Inc., and the University of California, Davis are collaborating on a project to revolutionize food safety by reducing the time it takes to identify the source of food-borne outbreaks from several days to only a few hours. On a global level, the technology will provide expanded testing to target specific bacteria and remove implicated food items intended for human consumption.

Agilent and UC Davis will develop MassCode PCR technology that integrates mass-tagged PCR primers for amplification and Liquid Chromatography/Mass Spectrometery for the rapid identification of Salmonella serovar subtypes as well as other food-borne pathogens. The technology combines the sensitivity and specificity advantages of PCR, with the partial automation of LC/MS, and the sensitivity of detection of the mass spectrometer. The mass tags are small organic molecules coupled to the PCR primers, which provide two unique identifiers per targetlike a dual barcode per target. These only appear in the MS analysis if the target was specifically and successfully amplified. By having two confirming signals per target, the likelihood of false positives is significantly decreased.

The techniques currently used to identify specific types of Salmonella were developed in the previous century," said Dr. Hailu Kinde, professor at the California Animal Health and Food Safety Laboratory System. They require a number of complex laboratory procedures, which are often laborious and can take up to a week to complete. In this era when food can be distributed across the globe in less than a day, it is critical that we have better tools to quickly identify the source of contamination before it spreads into the food supply. This exciting new project is a giant step toward reducing the time it takes to get test results and will make our food safer."